Services at the HTBC
The Stanford HTBC offers the following services to ALL Stanford researchers. Click here for rates. User Registration Form, Screeners Agreement Form
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Fully automated high-throughput screening (HTS) of Compound Libraries (130,000+ compounds) for both enzyme/protein-based assays and cell-based assays, using the Caliper Life Sciences Staccato system.
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Genomic siRNA screening with the siARRAY whole human genome siRNA library from ThermoFisher Scientific (formerly Dharmacon) targeting 21,000 genes. (now accepting proposals--Application)
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High-Content Screening using the ImageXpress Micro and image analysis using the MetaXpress software.
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High-Throughput Molecular Biology reagents and services, including access to cDNA libraries (Human ORFeome collection, 15,000 genes) and high-throughput cDNA purifications using the Qiagen BioRobot 3000.
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High-throughput assay development assistance with cell culture, experiment design, robotic programming and Standard Operating Procedure drafting.
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Screening data analysis assistance with protocols, hit determination and structure activity analyses using the MDL chemical database ISIS/HOST, Plate Manager, Assay Explorer and Report Manager.
Use of microplate reader detection systems, including Molecular Devices Analyst GT for fluorescence; fluorescence polarization; time-resolved fluorescence; absorbance and luminescence; the Flexstation II 384, for kinetic fluorescence reads to measure calcium mobilization and ion channels; and the EG&G Berthold LB96B dual injector luminometer.
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Use of liquid-handling robots, including the Sciclone ALH3000 (96- and 384-well pipetting), Velocity11 VPrep (96-well pipetting), Bio-Tek plate washers, Qiagen BioRobot 3000, Matrix Wellmate and Titertek Multidrop microplate dispensers, and Velocity11 PlateLoc plate heat sealer.
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Training for most of these services and much, much more!
Please contact us for more information.
