Fully automated high-throughput screening (HTS) of Compound Libraries for both enzyme/protein-based assays and cell-based assays, using the Caliper Life Sciences Staccato system.
Genomic siRNA screening with the siARRAY whole human genome siRNA library from ThermoFisher Scientific (formerly Dharmacon) targeting 21,000 genes. (now accepting proposals--Application)
High-Content Screening using the ImageXpress Micro and image analysis using the MetaXpress software.
High-Throughput Molecular Biology reagents and services, including access to cDNA libraries (Human ORFeome collection, 12,000 genes) and high-throughput cDNA purifications using the Qiagen BioRobot 3000.
High-throughput assay development assistance with cell culture, experiment design, robotic proramming and Standard Operating Procedure drafting.
Screening data analysis assistance with protocols, hit determination and structure activity analyses using the MDL chemmical database ISIS/HOST, Plate Manager, Assay Explorer and Report Manager.
Use of microplate reader detection systems, including Molecular Devices Analyst GT for fluorescence; fluorescence polarization; time-resolved fluorescence; absorbance and luminescence; the Flexstation II 384, for kinetic fluorescence reads to measure calcium mobilization and ion channels; and the EG&G Berthold LB96B dual injector luminometer.
Use of liquid-handling robots, including the Sciclone ALH3000 (96- and 384-well pipetting), Velocity11 VPrep (96-well pipetting), Bio-Tek plate washers, Qiagen BioRobot 3000, Matrix Wellmate and Titertek Multidrop microplate dispensers, and Velocity11 PlateLoc plate heat sealer.
Training for most of these services and much, much more!
