Human Genomic siRNA oligo Libraries at the HTBC
The HTBC is soliciting proposals for human genome wide small interfering RNA (siRNA) screens. The first round of RNAi (RNA interference) screening are ongoing. The second round of projects will commence in the fall, and will be chosen on a first validated/first run schedule.
Call for Proposal Flier (from October, 2006)
We have the siARRAY whole human genome siRNA library from ThermoFisher (formerly Dharmacon; press release). Stanford is also a member of RNAiglobal. The set consists of pools of 4 oligos per gene. In addition, we have the full genomic upgrade, consisting of each individual siRNA duplex (4 per gene). The set is divided into GPCR (517 genes), Kinase (779), Druggable genome (6022), and the remaining genome (13,804) for a total of 21,122 targeted genes.
Please contact us if you would like information on screening with the siRNA library. We are currently accepting proposals for RNAi screens. Please fill out the form and return to David E. Solow-Cordero, Ph.D.
HTBC siRNA Publications:
Paulsen, R.D., Soni, D.V., Wollman,R., Hahn, A.T., Yee, M.C., Guan, A., Hesley, J.A., Miller, S.C., Cromwell, E.F., Solow-Cordero, D.E., Meyer, T., Cimprich, K.A. A Genome-wide siRNA Screen Reveals Diverse Cellular Processes and Pathways that Mediate Genome Stability. Mol. Cell 35(2): 228-239 (2009). (PDF)
Resources for siRNA screeners:
MIACA - Minimum Information About A Cellular Assay
MIARE - Minimum Information About an RNAi Experiment
NIH Gene Data
RefSeq Human Genome Downloads
Harvard Screensaver
Ingenuity - Pathway analysis software
ID Converter - Gene Annotation Website
