High-Content Imaging

The **NEW** Molecular Devices ImageXpress Micro is located in CCSR 0116.

Please contact us for key card access to this room.

For high-content automated cellular imaging and analysis we are utilizing the Molecular Devices ImageXpress Micro with the live cell option. We use Semrock filters. See below for our filter configurations.

The IXMicro contains a fully automated inverted epifluorescence microscope that is capable of scanning a 6-, 24-, 96- or 384-well microplate and recording multiple fluorescent images in a few minutes. The ImageXpress Micro is then able to quantify morphological properties through the MetaXpress analysis software (modules). Our current ImageXpress Micro configuration permits the visualization of both fixed cells and live cells, as the instrument has a temperature-, humidity-, and carbon dioxide-controlled chamber. We plan to add an external automated incubator to permit long-term or large-scale studies of cellular events, and a robotic plate stacker, thereby increasing the throughput of this workstation.

ImageXpress Micro Schedule. Analysis Computer1, Analysis Computer2

To use the ImageXpress Micro, please contact David E. Solow-Cordero (x5-6002)

CFP/YFP/HcRed-3X-A Triple-band "Pinkel" Filter Set
Optimized for these fluorophores Cyan: CFP, AmCyan, SYTOX Blue, BOBO-1, BO-PRO-1 Yellow: YFP, Calcium Green-1, Fluo-3, Rhodamine 123 Red: HcRed, Cy3.5, Texas Red®, Alexa Fluor 594 Complete list of fluorophores for this filter set		Actual measured data from typical filters         Exciter 1 Dichroic Emitter ASCII Data ASCII Data ASCII Data Exciter 2 ASCII Data Exciter 3 ASCII Data
Set description This 5-filter "Pinkel" set is perfectly optimized for imaging CFP, YFP, and HcRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples triple-labeled with CFP, YFP, and an orange or red dye, like Texas Red. It is comprised of three single-band exciters along with a triple-band beamsplitter and a triple-band emitter, and thus works best with an exciter filter wheel or slider.
Filter Average Transmission Center Wavelengths Bandwidths *  Exciter 1 > 90% 427 nm 10 nm  Exciter 2 > 90% 504 nm 12 nm  Exciter 3 > 90% 589 nm 15 nm  Emitter > 90%, 90%, 90% 464, 542, 639 nm 23, 27, 42 nm   Average Reflection Reflection Bands Transmission Bands  Dichroic > 95% > 95% > 95% 420 - 430 nm 496 - 510 nm 579 - 596 nm 451 - 480 nm 530 - 561 nm 618 - 664 nm

DA/FI/TR/Cy5-4X-A Quad-band "Pinkel" Filter Set
Optimized for these fluorophores Blue: DAPI, Hoechst, AMCA, Alexa Fluor 350 Green: FITC, GFP, rsGFP, BODIPY, Alexa Fluor 488         Orange: TRITC, Cy3™, Texas Red®, MitoTracker Red,   Alexa Fluor 568 & 594 Red: Cy5™, APC, TOTO-3, TO-PRO-3, Alexa Fluor 647 & 660 Complete list of fluorophores for this filter set		Actual measured data from typical filters         Exciter 1 Dichroic Emitter ASCII Data ASCII Data ASCII Data Exciter 2 ASCII Data Exciter 3 ASCII Data Exciter 4 ASCII Data
Set description This 6-filter "Pinkel" quad-band set is designed for sequentially imaging DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This state-of-the-art set represents a break-through in quad-band performance, and has been tested by independent researchers to have up to four times the brightness, twice the signal-to-noise ratio and substantially lower crosstalk than any other such quad-band filter set on the market. The complete set is comprised of a quad-band beamsplitter and a quad-band emitter (these can be offered assembled in a microscope cube), plus four “no burn-out” single-band exciters that are intended to be mounted in an exciter filter wheel or slider.
Filter Average Transmission Center Wavelengths Bandwidths *  Exciter 1 > 85% 387 nm 11 nm  Exciter 2 > 90% 485 nm 20 nm  Exciter 3 > 90% 560 nm 25 nm  Exciter 4 > 90% 650 nm 13 nm  Emitter > 90%, 90%, 90%, 90% 440, 521, 607, 700 nm 40, 21, 34, 45 nm   Average Reflection Reflection Bands Transmission Bands  Dichroic > 95% > 95% > 95% > 95% 381 - 392 nm 475 - 495 nm 547 - 572 nm 643 - 656 nm 420 - 460 nm 510 - 531 nm 589 - 623 nm 677 - 722 nm

Old ImageXpress 5000A Webpage