Detection Systems at the HTBC (CCSR 0133)
| Plate Readers at the HTBC | Schedule | Data Sheet |
| Tecan Infinite M1000-multimode plate reader | Tecan Infinite M1000 (View Only) | Tecan Infinite M1000 |
| FLEXstation II-384-fluorescent plate reader | Flexstation II 384 (View Only) | Flexstation |
| Berthold LB 96 V Luminometer | Use Tecan | |
| AnalystGT-multimode plate reader | AnalystGT (View Only) | Analyst GT |
(use Mozzila Firefox if having problems with the calendars)
The Tecan Infinite M1000 is very flexible Quad4 monochromator-based 6 to 1536-well microplate reader and can measure;
- Absorbance (230 nm-1,000 nm)
- Fluorescence Intensity (Ex 230nm-850 nm, Em 280 nm-850 nm)
- Fluorescence Polarization (FP)
- Time-resolved fluorescence (TRF)
- Fluorescence resonance energy transfer (FRET)
- Homogeneous Time-Resolved Fluorescence (HTRF)
- Glow/Flash Chemiluminescence with Dual Injectors for both 96 and 384-well plates.
The HTBC Tecan Infinite M1000 has 50-microplate stackers and a Barcode Reader. It is extremely fast and sensitive and used primarily for high-throughput screens and platform specific applications (FP, TRF, HTRF), but can also be used for simple plate based absorbance reads and will replace the Berthold Luminometer for luminescence reads.
AnalystGT (Molecular Devices, Inc.)
The Analyst GT is very flexible filter-based 96-, 384-, and 1536-well microplate reader and can measure (see below for list of filters);
- Absorbance
- Fluorescence Intensity
- Fluorescence Polarization (FP)
- Time-resolved fluorescence (TRF)
- Fluorescence resonance energy transfer (FRET)
- Homogeneous Time-Resolved Fluorescence (HTRF)
- Chemiluminescence
The HTBC AnalystGT has 40-microplate stackers to allow its independent use, or it can be integrated with the Caliper LS Sciclone Staccato liquid handling system through a Caliper LS Twister II robotic arm. It is extremely fast and sensitive and used primarily for high-throughput screens in conjection with the Caliper LS Staccator system and platform specific applications (FP, TRF, HTRF)
FLEXstation II 384 (Molecular Devices, Inc.-Flex3 link-we don't have this)
The FLEXstation complements the Analyst GT, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, time-resolved fluorescence (TRF), fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. This enhanced capability allows for kinetic studies of biological events, such as G protein-coupled receptor activation (via Calcium mobilization, ie FLIPR screens) and ion channel gating in live cells. The FLEXstation also can read 6-, and 24-well plates for applications such as measuring cell migration. The FLEXstation is used primarily for platform specific applications, assay development and medium throughput screens.
Berthold LB 96 V luminometer has been replaced by the Tecan Infinite M1000
For luminescence studies, requiring injectors or long read times, we had a Berthold LB 96 V luminometer. The Tecan Infinite M1000 can perform all the same features with much greater sensitivity. The LB 96V instrument has two injectors for applications such as Dual luciferase (Firefly and Renilla measurements) . "The LB96V MicroLumat Plus is a universal microplate luminometer designed for all bio- and chemiluminescence measurements using 96-well or 384-well microplates or dot blots. Due to the use of up to 3 injectors, the measurement procedure can be adapted precisely to the chemical processes of the assay."
Note that all filters can be used for either emission or excitation with any dichrioc. Parentheses indicate current position in Emission or Excitation wheels.
