Detection Systems at the HTBC (CCSR 0133)

AnalystGT (Molecular Devices, Inc.)

The Analyst GT is very flexible filter-based 96-, 384-, and 1536-well microplate reader and can measure (see below for list of filters);

• Absorbance
• Fluorescence Intensity
• Fluorescence Polarization (FP)
• Time-resolved fluorescence (TRF)
• Fluorescence resonance energy transfer (FRET)
• Homogeneous Time-Resolved Fluorescence (HTRF)
• Chemiluminescence

The HTBC AnalystGT has 40-microplate stackers to allow its independent use, or it can be integrated with the Zymark Sciclone Staccato liquid handling system through a Zymark Twister II robotic arm. It is extremely fast and sensitive and used primarily for high-throughput screens and platform specific applications (FP, TRF, HTRF)

FLEXstation II 384 (Molecular Devices, Inc.)

The FLEXstation complements the Analyst GT, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, time-resolved fluorescence (TRF), fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. This enhanced capability allows for kinetic studies of biological events, such as G protein-coupled receptor activation (via Calcium mobilization, ie FLIPR screens) and ion channel gating in live cells. The FLEXstation also can read 6-, and 24-well plates for applications such as measuring cell migration. The FLEXstation is used primarily for platform specific applications, assay development and medium throughput screens.

For luminescence studies, requiring injectors or long read times, we have a Berthold LB 96 V luminometer. This instrument has two injectors for applications such as Dual luciferase (Firefly and Renilla measurements) . "The LB96V MicroLumat Plus is a universal microplate luminometer designed for all bio- and chemiluminescence measurements using 96-well or 384-well microplates or dot blots. Due to the use of up to 3 injectors, the measurement procedure can be adapted precisely to the chemical processes of the assay."

Note that all filters can be used for either emission or excitation with any dichrioc.