DA/FI/TR/Cy5-4X4M Filter Set – Fluorophores

 

Table of fluorophores that may be used with the BrightLine® DA/FI/TR/Cy5-4X4M filter set:  Note that results may vary depending upon instrumentation and conditions.

 
Blue Fluorophore
Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 1,8-ANS
372
480
 4-methylumbelliferone
360
440
 7-amino-4-methylcoumarin
351
430
 7-hydroxy-4-methylcoumarin
360
449
 ABQ
344
445
 Acridine
362
462
 Alexa Fluor 350™
346
442
 AMCA (Aminomethylcoumarin)
345
425
 AMCA-X
353
442
 Aminocoumarin
350
445
 Anthrocyl stearate
370
446
 Beta lactamase
409
450
 BFP (Blue Fluorescent Protein)
382
448
 Bisbenzimide (Hoechst)
360
461
 Blancophor FFG
390
470
 Blancophor SV
370
435
 Calcein Blue
373
440
 Calcofluor White
395
440
 Cascade Blue™
377
420
 Catecholamine
410
470
 Coumarin Phalloidin
387
470
 CPM Methylcoumarin
384
469
 DAPI
345
455
 EBFP
380
440
 Fast Blue
360
440
 FIF (Formaldehyde Induced Fluorescence)
405
433
 Gloxalic Acid
405
460
 Granular Blue
355
425
 Hoechst 33258
345
487
 Hoechst 33342
347
483
 Hoechst 34580
392
440
 HPTS
355
465
 Indo-1
346
475
 Intrawhite Cf 
360
430
 Laurodan 
355
460
 Leucophor PAF, SF, WS
370
430
 LysoSensor Blue
374
424
 LysoSensor Blue DND-167  
374
425
 Lysosensor Blue DND-192
374
424
 LysoTracker Blue
373
422
 Mag-Indo-1
349
480
 Marina Blue
365
460
 Methoxycoumarin
360
410
 Monochlorobimane
380
461
 Pacific Blue™
405
455
 PBFI
360
420
 Phorwite AR
360
430
 Phorwite BKL
370
430
 Phorwite Rev
380
430
 Phorwite RPA
375
430
 SBFI
360
420
 sgBFP™
387
450
 SITS (Primuline)
410
450
 SITS (Stilbene Isothiosulphonic Acid)
365
460
 SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium)
344
443
 Thioflavin TCN
350
460
 Thiolyte
378
483
 Tinopol CBS (Calcofluor White)
390
430
 True Blue
365
425
 Uvitex SFC
365
435
 Y66H
360
442
 
Green Fluorophore
Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 5-carboxy-2,7-dichlorofluorescein
504
529
 5-FAM (5-carboxyfluorescein)
492
518
 6-FAM
492
515
 Acridine Orange
500
526
 Acridine Orange + DNA
502
526
 Acridine Yellow
470
550
 Alexa Fluor 488™
495
519
 Astrazon Orange R
470
540
 ATTO-TAG™ CBQCA
465
560
 Auramine
460
550
 BODIPY
505
513
 BODIPY 492/515
490
515
 BODIPY 505/515
502
510
 BTC
464
530
 Calcein
494
517
 CFDA
494
520
 CMFDA
494
520
 Cyclic AMP Fluorosensor (FiCRhR)
500
517
 DCFDA
504
529
 DTAF
494
520
 FAM
492
518
 FDA
494
520
 FITC (Fluorescein)
492
520
 Fluo-4
494
516
 Fluoro-Emerald
495
524
 FluorX
494
520
 GFP (EGFP)
489
508
 JC-1
498
525
 JC-9
506
525
 LIVE/DEAD Kit Animal Cells
494
517
 LysoTracker Blue-White
466
536
 LysoTracker Green
504
511
 mHoneydew
478
561
 MitoTracker™ Green
490
516
 NBD
466
539
 NBD-X
467
538
 NeuroTrace 500/525 Green
497
524
 NeuroTrace 500/525 Green Fluorescent Nissl Stain
500
525
 Nissl
497
524
 Oregon Green™
503
522
 Oregon Green™ 488
492
517
 Oregon Green™ 500
497
517
 Oregon Green™ 514
506
526
 PKH67
496
520
 Rhod-123
507
529
 Rhodamine 110
496
520
 Rhodol Green
496
523
 rsGFP (red shifted GFP, S65T)
498
516
 Sodium Green
506
532
 SYTO Green Fluorescent Nucleic Acid Stains 12, 13, 16, 21, 23, 24
494
515
 SYTOX Green
504
523
 
Orange Fluorophore
Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 5-TAMRA (5-carboxytetramethylrhodamine, high pH > 8)
542
568
 Alexa Fluor 532™
532
553
 Alexa Fluor 568™
494
520
 Alexa Fluor 594™
494
520
 Alizarin Red
540
580
 Astrazon Brilliant Red 4G
500
585
 BODIPY 542/563
543
563
 BODIPY TMR
542
574
 BO-PRO™ -3
544
570
 Cy3™
552
570
 DiI
551
565
 DiI (DiIC18(3))
549
565
 Ethidium Bromide
510
595
 Genacryl Brilliant Red B
520
590
 HEX
535
556
 JOE
525
555
 MitoTracker Red
494
520
 NED
546
575
 Nile Red
535
590
 PKH26 (Sigma)
551
567
 POPO-3
533
574
 PO-PRO-3
539
567
 Rhod-2
552
576
 Rhodamine
550
573
 Rhodamine BB
540
580
 Rhodamine BG
540
572
 Rhodamine Phalloidine
542
565
 Rose Bengal
533
575
 Sevron Brilliant Red 2B
520
595
 Sevron Brilliant Red B
530
590
 SpectrumGold
530
555
 Sulphorhodamine B can C
520
595
 Texas Red®
 TMR
542
574
 TRITC
545
563
 Xylene Orange
546
580
 
Red Fluorophore
Peak Excitation
Wavelength* (nm)
Peak Emission
Wavelength* (nm)
 Alexa Fluor 647™
647
666
 Alexa Fluor 660™
663
690
 APC (Allophycocyanin)
651
661
 BODIPY 650/665 Dye
650
665
 BODIPY 650/665-X
647
665
 Cy5™
649
666
 DiD (DiIC18(5)) - Lipophilic Tracer
644
665
 DY-635-NHS
634
664
 Indodicarbocyanine (DiD)
644
665
 SYTO Red Fluorescent Nucleic Acid Stains 60, 62, 63
655
675
 Thiadicarbocyanine (DiSC3)
651
674
 TOTO-3, TO-PRO-3
642
661
 Ultralite
656
678
 
* These are typical wavelengths to be used for guidance purposes only. Actual peak wavelengths may vary for different applications.