Instrumentation available at the HTBC (CCSR 0133)
The HTBC has instrumentation for high-throughput (96 and 384 well microplate) liquid handling, high-throughput detection and high-content screening. Our small molecule HTS liquid handling system includes an integrated robotic arm for fully automated enzyme/protein and cell-based screening, while our siRNA liquid handling system allows for fully automated siRNA transfections, cell fixing and staining, and high content imaging. For more information select one of the tabs on the left. The Caliper LS Staccato small molecule liquid handling system and the Analyst GT were purchased with NCRR NIH Instrumentation grant number S10RR019513. The upgraded Agilent (formerly Velocity11) siRNA Screening system and advanced automated options for the ImageXpress Micro were purchased with NCRR NIH Instrumentation grant number S10RR026338.
See video of our integrated system running a real small molecule screen.
PART 1 : Cell plate comes from automated Incubator, lid is removed, Twister picks up plate and takes to bar code reader.
PART 2 : Cell plate is removed from multidrop liquid dispenser and placed on Sciclone deck.
PART 3 : 100 nL of each compound is transfered from compound plate to cell plate with the 384 Pin Tool.
Steve Gladfelter/VAS
Tecan Infinite M1000 schedule (multimode plate reader).
Flexstation II 384 schedule (fluorescent intensity and kinetic experiments).
AnalystGT schedule (use Tecan Infinite M1000 now, contact us for more info.)
Luminometer schedule (use Tecan Infinite M1000 now, contact us for more info.)
Liquid Handling Robots:
Multidrop/Wellmate/PlateWasher schedule.
Velocity11 VPrep/Aglient System/Plate Sealer schedule.
High Content Imager:
ImageXpress Micro check schedule HERE.
Analysis Computer1, Analysis Computer2, IX Analysis3126 (no longer available)
The funding for the purchase of most of our instrumentation was provided by the NIH Shared Instrumentation Grant numbers S10RR019513 and S10RR026338, and by an anonymous donation.
