DAVID E. SOLOW-CORDERO, Ph.D.
Department of Chemical & Systems Biology
Stanford University School of Medicine
EDUCATION:
Ph.D., 1995, Department of Molecular and Cellular Biology
University of California at Berkeley
Thesis Title: In vitro Analysis of Rifampicin Resistant Escherichia coli RNA Polymerases
Michael J. Chamberlin, Professor
University Fellowship, September 1991 to July 1992
BS, 1990, Department of Biology
Massachusetts Institute of Technology
Anthony J. Sinskey, Professor
National Hispanic Scholar, September 1986 to May 1987
EMPLOYMENT HISTORY:
Stanford University School of Medicine
Department of Chemical & Systems Biology
September 2003 to Present
Responsibilities include:
· Select, purchase, program, and maintain laboratory robotics and detection systems including a fully automate Caliper Life Sciences Staccato cell based system (Sciclone ALH3000, Twister II, automated incubator), a Velocity11 VPrep/BenchCel system and Molecular Devices ImageXpress, AnalystGT, and Flexstation 384.
· Select and purchase chemical libraries to be used for screening.
· Identify and manage research collaborations with Stanford faculty and students to incorporate high-throughput technologies in their research programs.
· Build, develop and manage the ORACLE based MDL cheminformatics data systems (ChemBioAE, Plate Manager and Assay Explorer).
· Schedule and manage HTBC group including Scientists and Research Associates. Oversee all financial and administrative tasks of the HTBC, a Stanford service center.
· Manage human whole genome RNA interference screens
· Stanford University representative to the RNAi Global Initiative.
Ceretek, LLC., South San Francisco, CA
Principal Scientist, High Throughput Screening (HTS) and Informatics
June 1999 to September 2003
Responsibilities include:
· Design, develop, scale-up, and validate G-Protein Coupled Receptor assays for HTS
· Select, purchase, program, and maintain laboratory robotics including a Molecular Devices FLIPR384 and a Beckman Multimek.
· Select and purchase chemical libraries to be used for HTS.
· Schedule and manage HTS group including Scientists and Research Associates.
· Manage collaborations with chemists and biologists concerning screening data and lead identification.
· Build, develop and manage MDL cheminformatics data system (ISIS Base/Host and ORACLE).
FibroGen, Inc., South San Francisco, CA
Scientist, Enzymology
July 1995 to June 1999
Responsibilities include:
· Design, develop, and scale-up assays for HTS
Including, characterization and purification of procollagen/collagen processing enzymes, development of robust scaleable assay to aid in the discovery of novel inhibitors of fibrosis.
· Select, purchase, program, and maintain laboratory robotics.
· Schedule and manage HTS group including Scientists and Research Associates.
· Build, develop and manage MDL cheminformatics data system (ISIS Base/Host and ORACLE).
University of California at Berkeley
Graduate Student
Thesis Advisor: Michael J. Chamberlin, Professor
September 1990 to June 1995
· Research on In vitro analysis of rifampicin resistant E. coli RNA polymerases.
Rotation projects:
· Research on protein membrane translocation in yeast. Randy Schekman, Professor
· Research on enzyme substrate channeling in yeast. Jack Kirsch, Professor
Massachusetts Institute of Technology
Undergraduate Researcher
Advisor: Anthony J. Sinskey, Professor
May 1988 to August 1990
· Research on the allosteric inhibition by threonine on homoserine dehydrogenase in C. glutamicum.
Bayer, Inc., Elkhart, Indiana
Research Assistant
May 1987 to August 1987
· Research on Aspergillus Niger transformation.
Bayer, Inc., Elkhart, Indiana
Laboratory Assistant
May 1986 to August 1986
HONORS AND AWARDS:
1986-1987 National Hispanic Scholar, MIT
1991-1992 University Fellowship, University of California at Berkeley
2005-2007 Study Section, Solicitation of Assays for High Throughput Screening (HTS) in the Molecular Libraries Screening Centers Network (NIH, MLSCN)
2006-2007 Member, Education Committee, Society of Biomolecular Sciences (SBS)
2009 Study Section, Instrumentation and Systems Development, Bioengineering Sciences & Technologies Integrated Review Group, Temporary Member (NIH, CSR)
2009 Study Section, Assay Development for High-Throughput Molecular Screening (NIH, CSR)
PUBLICATIONS AND PATENTS:
Hyman, J., Firestone, A., Heine, V., Zhao, Y., Ocasio, C., Han, K., Sun, M., Rack, P., Sinha, S., Wu, J., Solow-Cordero, D., Jiang, J., Rowitch, D., and Chen, J. Small-molecule inhibitors reveal multiple strategies for Hedgehog pathway blockade. Proc.Natl.Acad.Sci.USA. Aug 18;106(33):14132-7. Epub Aug 5 (2009).
Paulsen, R.D., Soni, D.V., Wollman,R., Hahn, A.T., Yee, M.C., Guan, A., Hesley, J.A., Miller, S.C., Cromwell, E.F., Solow-Cordero, D.E., Meyer, T., Cimprich, K.A. A Genome-wide siRNA Screen Reveals Diverse Cellular Processes and Pathways that Mediate Genome Stability. Mol. Cell 35(2): 228-239 (2009).
Chan C.T., Paulmurugan R., Reeves R.E., Solow-Cordero D., Gambhir S.S. Molecular Imaging of Phosphorylation Events for Drug Development. Mol Imaging Biol. May-Jun;11(3): 144-58 (2009). Epub Dec 2 (2008).
Solow-Cordero, D.E. Academia catching up to industry: How liquid handling is enhancing basic research. G.I.T. Laboratory Journal 3-4: 64-65 (2007).
Lee, S., Solow-Cordero, D.E., Kessler, E., Takahara, K., and Greenspan, D.S. Transforming growth factor-b regulation of bone morphogenetic protein-1/procollagen C-proteinase and related proteins in fibrogenic cells and keratinocytes. J. Biol. Chem. 272: 19059-19066 (1997).
Altmann, C.R.; Solow-Cordero, D.E.; Chamberlin, M.J.: RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in a binary enzyme-RNA complex. Proc.Natl.Acad.Sci.USA. 91: 3784-3788 (1994).
Archer, J.A.; Solow-Cordero, D.E.; Sinskey, A.J.: A C-terminal deletion in Corynebacterium glutamicum homoserine dehydrogenase abolishes allosteric inhibition by l-threonine. Gene. 107: 53-59 (1991).
Twelve filed US patent applications: Solow-Cordero, D. Shankar, G., J.V. Spencer, and C. Gluchowski. Methods of treating conditions associated with Edg receptors.
One issued US Patent: Solow-Cordero, D. Shankar, G., J.V. Spencer, and C. Gluchowski. Methods of treating conditions associated with an Edg-3 receptor. 7,208,502 April 24, 2007,
Two International patent applications:
Shankar, G., Solow-Cordero, D., J.V. Spencer, and C. Gluchowski. Methods of treating conditions associated with an EDG receptor. WO 2003/062392, July 31, 2003.
Solow-Cordero, D., Shankar, G., J.V. Spencer, and C. Gluchowski. Methods of treating conditions associated with an EDG-1 receptor. WO 2004/009816, January 29, 2004.
INVITED LECTURES:
Laboratory Robotics Interest
Group (LRIG)-Bay Area Meeting: Current Bottlenecks in Laboratory Automation
15 March 2005, Burlingame, California
Society for Biomedical
Screening (SBS) West Coast Regional Meeting: Exploiting the Druggable Genome:
21-22 April 2005, San Mateo, California
Elsevier MDL Biology Data Management Exchange
5-6 May 2005, Scottsdale, Arizona
RNAiGlobal-Dharmacon
25-26 September 2006, Amsterdam, The Netherlands
RNAiGlobal-ThermoFisher
12-13 April 2007, Boulder, Colorado
27-28 September 2007, Barcelona, Spain
2-4 April 2008, Boston, Massachusetts
22-23 September 2009, Dublin, Ireland
TEACHING
University of California at Berkeley
September to December 1994, Instructor for Undergraduate Seminar: Novel Reactions in Transcription Regulation (MCB 119)
January to May 1993, Teaching Assistant for Introduction to Biology (MCB 15)
November 1991 to February 1992, Teaching Assistant for Biochemistry Lab (MCB 110L)
Stanford University
20 April 2005, Guest Lecturer, Drug Discovery (MolPharm 240)
ASSOCIATIONS
Society for Biomolecular Sciences (SBS)
Association of Laboratory Automation (ALA)
AAAS