DAVID E. SOLOW-CORDERO, Ph.D.

Director, High-Throughput Bioscience Center (HTBC)

Department of Chemical & Systems Biology

Stanford University School of Medicine

 

EDUCATION:

Ph.D., 1995, Department of Molecular and Cellular Biology

University of California at Berkeley

Thesis Title: In vitro Analysis of Rifampicin Resistant Escherichia coli RNA Polymerases

Michael J. Chamberlin, Professor

University Fellowship, September 1991 to July 1992

 

BS, 1990, Department of  Biology

Massachusetts Institute of Technology

Anthony J. Sinskey, Professor

National Hispanic Scholar, September 1986 to May 1987

 

EMPLOYMENT HISTORY:

Stanford University School of Medicine

Department of Chemical & Systems Biology

Director, High-Throughput Bioscience Center (HTBC)

September 2003 to Present

Responsibilities include:

·        Select, purchase, program, and maintain laboratory robotics and detection systems including a fully automate Caliper Life Sciences Staccato cell based system (Sciclone ALH3000, Twister II, automated incubator), a Velocity11 VPrep/BenchCel system and Molecular Devices ImageXpress, AnalystGT, and Flexstation 384.

·        Select and purchase chemical libraries to be used for screening.

·        Identify and manage research collaborations with Stanford faculty and students to incorporate high-throughput technologies in their research programs.

·        Build, develop and manage the ORACLE based MDL cheminformatics data systems (ChemBioAE, Plate Manager and Assay Explorer).

·        Schedule and manage HTBC group including Scientists and Research Associates.  Oversee all financial and administrative tasks of the HTBC, a Stanford service center.

·        Manage human whole genome RNA interference screens

·        Stanford University representative to the RNAi Global Initiative.

 

Ceretek, LLC., South San Francisco, CA

Principal Scientist, High Throughput Screening (HTS) and Informatics

June 1999 to September 2003

Responsibilities include:

·        Design, develop, scale-up, and validate G-Protein Coupled Receptor assays for HTS

·        Select, purchase, program, and maintain laboratory robotics including a Molecular Devices FLIPR384 and a Beckman Multimek.

·        Select and purchase chemical libraries to be used for HTS.

·        Schedule and manage HTS group including Scientists and Research Associates.

·        Manage collaborations with chemists and biologists concerning screening data and lead identification.

·        Build, develop and manage MDL cheminformatics data system (ISIS Base/Host and ORACLE).

 

FibroGen, Inc., South San Francisco, CA

Scientist, Enzymology

July 1995 to June 1999

Responsibilities include:

·        Design, develop, and scale-up assays for HTS

Including, characterization and purification of procollagen/collagen processing enzymes, development of robust scaleable assay to aid in the discovery of novel inhibitors of fibrosis.

·        Select, purchase, program, and maintain laboratory robotics.

·        Schedule and manage HTS group including Scientists and Research Associates.

·        Build, develop and manage MDL cheminformatics data system (ISIS Base/Host and ORACLE).

 

University of California at Berkeley

Graduate Student

Thesis Advisor:  Michael J. Chamberlin, Professor

September 1990 to June 1995

·        Research on In vitro analysis of rifampicin resistant E. coli RNA polymerases.

Rotation projects:

·        Research on protein membrane translocation in yeast.  Randy Schekman, Professor

·        Research on enzyme substrate channeling in yeast.  Jack Kirsch, Professor

 

Massachusetts Institute of Technology

Undergraduate Researcher

Advisor:  Anthony J. Sinskey, Professor

May 1988 to August 1990

·        Research on the allosteric inhibition by threonine on homoserine dehydrogenase in C. glutamicum.

 

Bayer, Inc., Elkhart, Indiana

Research Assistant

May 1987 to August 1987

·        Research on Aspergillus Niger transformation.

 

Bayer, Inc., Elkhart, Indiana

Laboratory Assistant

May 1986 to August 1986

 

HONORS AND AWARDS:

1986-1987          National Hispanic Scholar, MIT

1991-1992          University Fellowship, University of California at Berkeley

2005-             Member, Study Section “Solicitation of Assays for High Throughput Screening (HTS) in the Molecular Libraries Screening Centers Network (NIH, MLSCN) ”

2006-             Member, Education Committee, Society of Biomolecular Sciences (SBS)

 

 

PUBLICATIONS AND PATENTS:

Twelve filed US patent applications:  Solow-Cordero, D. Shankar, G., J.V. Spencer, and C. Gluchowski.  Methods of treating conditions associated with Edg receptors. 

One issued US Patent:  Solow-Cordero, D. Shankar, G., J.V. Spencer, and C. Gluchowski.  Methods of treating conditions associated with an Edg-3 receptor. 7,208,502 April 24, 2007,

 

Two International patent applications:

Shankar, G., Solow-Cordero, D., J.V. Spencer, and C. Gluchowski.  Methods of treating conditions associated with an EDG receptor.   WO 2003/062392, July 31, 2003. 

Solow-Cordero, D., Shankar, G., J.V. Spencer, and C. Gluchowski.  Methods of treating conditions associated with an EDG-1 receptor.   WO 2004/009816, January 29, 2004.

 

Solow-Cordero, D.E. Academia catching up to industry:  How liquid handling is enhancing basic research.  G.I.T. Laboratory Journal 3-4: 64-65 (2007).

 

Lee, S., Solow-Cordero, D.E., Kessler, E., Takahara, K., and Greenspan, D.S. Transforming growth factor-b regulation of bone morphogenetic protein-1/procollagen C-proteinase and related proteins in fibrogenic cells and keratinocytes. J. Biol. Chem. 272:  19059-19066 (1997).

 

Altmann, C.R.; Solow-Cordero, D.E.; Chamberlin, M.J.: RNA cleavage and chain elongation by Escherichia coli DNA-dependent RNA polymerase in a binary enzyme-RNA complex. Proc.Natl.Acad.Sci.USA. 91:  3784-3788 (1994).

 

Archer, J.A.; Solow-Cordero, D.E.; Sinskey, A.J.: A C-terminal deletion in Corynebacterium glutamicum homoserine dehydrogenase abolishes allosteric inhibition by l-threonine. Gene. 107:  53-59 (1991).

 

 

INVITED LECTURES:

Laboratory Robotics Interest Group (LRIG)-Bay Area Meeting:  Current Bottlenecks in Laboratory Automation
15 March 2005, Burlingame, California

 

Society for Biomedical Screening (SBS) West Coast Regional Meeting:  Exploiting the Druggable Genome:
21-22 April 2005,
San Mateo, California

 

Elsevier MDL Biology Data Management Exchange

5-6 May 2005, Scottsdale, Arizona

 

RNAiGlobal-Dharmacon

25-26 September 2006, Amsterdam, The Netherlands

 

RNAiGlobal-ThermoFisher

12-13 April 2007, Boulder, Colorado

 

TEACHING

University of California at Berkeley

September to December 1994, Instructor for Undergraduate Seminar:  Novel Reactions in Transcription Regulation (MCB 119)

January to May 1993, Teaching Assistant for Introduction to Biology (MCB 15)

November 1991 to February 1992, Teaching Assistant for Biochemistry Lab (MCB 110L)

 

Stanford University

20 April 2005, Guest Lecturer, Drug Discovery (MolPharm 240)

 

ASSOCIATIONS

Society for Biomolecular Sciences (SBS)

Association of Laboratory Automation (ALA)

AAAS